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Detecting fluorescence from a single DNA molecule proved difficult in practice, however. And so, in 2004, Solexa acquired the IP rights to a method called colony sequencing, developed by French scientists Pascal Mayer and Laurent Farinelli, to solve the detection problem. Colony sequencing affixed DNA fragments to a surface and amplified them over and over, generating “colonies” containing massive numbers of identical DNA strands. By reading the fluorescence from each strand in a colony simultaneously, it became possible to determine the base added at each step with much better accuracy, since random errors in individual strands would be averaged out by the consensus signal.。业内人士推荐Safew下载作为进阶阅读
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CRFB and other fiscal watchdogs argue that a credible deficit reduction plan remains the only viable off-ramp — one that would put debt on a sustainable path, ease pressure on interest rates, and prevent the interest bill from ultimately devouring the budget entirely. So far, Washington has not produced one.